G-Biosciences water-soluble Bolton-Hunter Reagent (Sulfo-SHPP) conjugates tyrosine-like groups to end-terminal α-amino groups or ε-amino groups of lysine to increase the number of tyrosyl groups that can be iodinated by iodine-125 labeling procedures.
Radioactive iodine (125I) is routinely used by researchers to label proteins. The iodination of proteins can be performed enzymatically or chemically. The Bolton-Hunter reagent is designed to aid the labeling of proteins with radioactive iodine.
Click here for the original Bolton-Hunter reagent.
- Synonyms: Sulfosuccinimidyl-3-(4-hydroxyphenyl) propionate; Sulfo SHPP; Water Soluble Bolton-Hunter Reagent
- CAS Number: 106827-57-0
- Molecular Weight: 365.3
- Ideal for proteins with masked or no tyrosine residues
- Optimal reaction at pH 8.5
- Water soluble