Superior™ Blocking Buffer

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SKU: 786-601-XX Category:
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Description

Superior™ Blocking Buffer contains a proprietary antiginically non-determinant protein for blocking non-specific sites during ELISA, membrane blotting, immunohistochemistry and other applications. 

 

Superior™ Blocking Buffer is ideal for a high signal to background ratio in most system. Superior™ Blocking Buffer uses a non-serum protein and does not contain biotin or other animal source proteins to interfere with immuno-complexes. Superior™ Blocking Buffer is suitable for assays that use avidin/streptavidin systems.

 

Superior™ Blocking Buffer for Precipitating Substrate is a modification of Superior™ Blocking Buffer. This blotting buffer has been optimized for used in blotting protocols that use precipitating substrates, such as TMB (3, 3′, 5, 5′-Tetramethylbenzidine) and BCIP (5-Bromo-4-Chloro-3′-Indolyphosphate p-Toluidine Salt) and NBT (Nitro-Blue Tetrazolium Chloride) substrates. Superior™ Blocking Buffer for Precipitating Substrate is not suitable for ELISA or immunohistochemistry staining.

 

Available in multiple formats using TBS, PBS, TBS with 0.05% Tween®-20 or PBS with .05% Tween®-20.  Also supplied as a convenient dry form that is stable at room temperature. Each dry format pack is makes 200ml Superior™ Blocking Buffer.

 

Features

  • Non serum protein blocking agent
  • Rapid blocking times; ~2 minutes for ELISA
  • Multiple formats; ready-to-use liquid (500ml) or dry buffer packs (for 5 x 200ml)
  • Available with and without detergent (Tween®-20)

 

Applications

  • For blocking Western blot membranes (PVDF and nitrocellulose)
  • For blocking and storage of ELISA plates
  • For blocking prior to immunohistochemistry staining

 

SuperiorBlocking Buffer for Precipitating Substrate is not suitable for ELISA or immunohistochemistry staining.

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