A Chemically Modified, TPCK treated, Affinity Purified Trypsin
Trypsin is a serine endopeptidase that specifically cleaves peptide bonds on the carboxy side of s-aminoethyl cysteine, arginine and lysine residues, and typically, there is little to no cleavage at arginyl-proline and lysyl-proline bonds. The distribution of these residues in proteins allows trypsin digestion to produce peptides that are readily identified by mass spectrometry.
Native trypsin is prone to autolysis that results in pseudotrypsin, which exhibits a broader proteolytic specificity (a chymotrypsin-like activity) and trypsin fragments that interfere with sequence analysis.
G-Biosciences’ Mass Spectrometry Grade Trypsin is chemically methylated to yield an enzymatically active protein with maximum trypsin specificity and extreme resistance to autolysis. In addition, the modified trypsin is TPCK treated to inactive the interfering chymotrypsin activity, and the resulting protein is affinity purified and lyophilized to produce our Mass Spectrometry Grade Trypsin.
The resulting trypsin has a specific activity over 10000 units per milligram protein. The maximum activity is in the pH range of 7 to 9 and activity is reversibly inactivated at a pH of 4.
Unlike other trypsin preparations, our Mass Spectrometry Grade Trypsin is highly stable. As a result, it can be stored for a long period of time without any loss of activity (Figure 2).
We supply two sources of Mass Spectrometry Grade Trypsin, either bovine or porcine. For mass spectrometry sequence analysis, a trypsin to protein ratio ranging from 1:20 to 1:100 is recommended. For convenience, our porcine Mass Spectrometry Grade Trypsin is supplied lyophilized as 20, 100 or 200µg vials with a specific resuspension buffer. Our bovine Mass Spectrometry Grade Trypsin is supplied lyophilized as 20µg vials with a specific resuspension buffer.
- Affordable Mass Spec Grade TrypsinUltra pure porcine or bovine trypsin
- Modified by methylation and TPCK treatment
- Resistant to autolysis and degradation
- Stable at ambient temperature and suitable for long term storage
- Specific activity: >10000U/mg protein
- Digestion of proteins for sequence and peptide fragment analysis
- Suitable for sequencing and mass spectrometry applications