WEB`s Plus Protein Ladder PS11 prestained,11bands,10 -175kD

Key Features

Protein Molecular Weight Marker PS11 (prestained) is ideal for precise sizing of proteins by SDS-PAGE and for Western blotting.

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SKU: BI-3100XX Categories: ,
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Description

Proteinleiter - Proteinladder von GeneON

Description/Preparation:
Protein Marker PS11 contains 11 proteins that resolve into sharp, tight bands in the range of 10-175 kDa. The Protein Ladder allows to monitor molecular weight separation during electrophoresis, estimate molecular weights The marker is ready-to-use. There is no need to boil. Easy to identify: includes the ~10, ~40 and ~90 kDa reference bands coupled with an blue dye

 

Applications
• Monitoring of protein migration during SDS-

   polyacrylamide gel electrophoresis.
• Monitoring of protein transfer onto membranes
   during Western blotting.
• Sizing of proteins on SDS-polyacrylamide gels
   and Western blots.

Usage:  
Mini gel application: 5 μl/well; 2.5 µl per well blots
Standard gel application: 10 μl/well; 5 µl per well for blots

Number of bands: 11      10.5, 14, 22, 29, 42, 51, 62, 70, 95, 130, 175 kDa
 

 

Protocol /Stability tests / Quality Tests

Loading: 
Loading Denaturing Polyacrylamide gels (SDS-PAGE):
– Thaw marker at room temperature or heat at 37 –   40 °C for a few minutes. Do not boil!
– Vortex gently to ensure the solution is 
  homogeneous and load the ladder on SDS-
  polyacrylamide gel
– 5 μl per well for mini-gels, 2.5 μl per well for blots
– 10 μl per well for large gels, 5 μl per well for blots

 

1 ml marker is sufficient for 200 mini gels or 100 standard gels.
It is recommended to divide the marker into aliquots to avoid contamination of the stock solution.

 

 

Additional information

Quantity

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