Well-Coated™ Biotin

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SKU: 786-747-XX Category:
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Description

Well-Coated™ Biotin plates are designed to specifically bind avidin, streptavidin or Neutravidin™ conjugated molecules, including enzyme conjugates. 

 

Biotin exhibits an extraordinary binding affinity for avidin (Ka=1015M-1) and streptavidin (Ka=1015 M-1).  Biotin and avidin interaction is rapid and once the bond is established it can survive up to 3M guanidine-hydrochloride and extremes of pH. Biotin-avidin bonds can only be reversed by denaturing the avidin protein molecule with 8M guanidine-hydrochloride at pH1.5 or by autoclaving.  Streptavidin and Neutravidin™ in many respects are similar to avidin except that they have  no carbohydrate and their solubility in aqueous buffer is much lower than avidin.  Neutravidin™ also lacks the RYD sequence eliminating interaction with RGD domain of adhesion receptors.   The binding of strepatavidin and Neutravidin™ is similar to that of avidin, but with less non-specific binding. 

 

The wells are coated to a 200µl depth and are supplied pre-blocked in our proprietary Superior™ Blocking Buffer.  The plates have the capacity to bind 30-50ng streptavidin per well.  

 

The clear, white and black plates are offered for colorimetric, chemiluminescence and fluorescent detection systems, respectively.
 

 

Features

  • Binds avidin, streptavidin and Neutravidin™ conjugated molecules
  • Reduced non-specific binding as plates are pre-blocked with Superior™ Blocking Buffer

 

 

Additional information

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