The XIT™ Genomic DNA from Tissue kit is designed for the isolation of genomic DNA from fresh, frozen or methanol/ acetone fixed tissues.
The kit uses three main steps (see figure) that are rapid & efficient cell lysis, enzymatic protein digestion and complete protein precipitation, followed by DNA precipitation. This yields high quality genomic DNA.
The kit is supplied with three protocols for the isolation of:
1-10mg fresh or frozen tissue
50-100mg fresh or frozen tissue
5-10mg fixed tissue
Three sizes of kit are available for processing a total of 0.25, 2.5 and 10g of tissue. The purified DNA has a A260/A280 ratio between 1.7 and 1.9 and is up to 200kb in size. The yield is approximately 0.5-10µg/mg tissue.
Isolate high quality genomic DNA
Suitable for fresh, frozen and fixed tissue
Yield 0.5-10µg/mg tissue
Suitable for archive quality DNA.