XIT™ Genomic DNA from Yeast

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SKU: 786-348-XX Category:
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Description

The XIT Genomic DNA from Yeast kit is designed for the isolation of genomic DNA from overnight yeast cultures.
 
The kit uses three main steps (see figure) that are rapid & efficient enzymatic digestion of cell walls, spheroplast lysis and complete protein precipitation, followed by DNA precipitation. This yields high quality genomic DNA.
 
Two sizes of kit are available for processing a total of 25 and 250ml of bacterial culture.  XIT Genomic DNA from Yeast Kit protocol is designed to use 1ml overnight culture, however the protocol can be easily adapted for larger tissue sample sizes. The purified DNA has a A260/A280 ratio between 1.7 and 1.9 and is up to 200kb in size. The yield is approximately 1-6µg/ml culture.
 

Features

  • Isolate high quality genomic DNA
  • Suitable for Yeast cultures
  • Yield 1-6µg/mg tissue
  • Suitable for archive quality DNA.

 

 

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