The rAmylase Project
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Assaying for Amylase Activity (Lab 6C/6D)
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Characterizing Proteins by PAGE (Lab 5F)
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Confirming the Amylase gene using PCR (Lab 13h)
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Determining the Concentration of Amylase (Lab 7D)
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Direct ELISA of Bacterial alpha Amylase (Lab 6E)
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Restriction Digestion to Verify the p-Amylase Plasmid (Lab 8b)
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Transformation of E. coli with p-Amylase (Lab 8C)
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Using a Miniprep Kit for p-Amylase Plasmid Isolation (Lab 8G)
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Using Ion Exchange Chromatography to Separate Proteins (Lab 9C)
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Western Blot to Identify Alpha-Amylase (Lab 6G)